Escherichia coli T13

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli W2637

Escherichia coli W3110

Escherichia coli T8

Escherichia coli T9

Escherichia coli T10

Escherichia coli T11

Escherichia coli T13

Derived strains

Genotype with respect to parental

ΔTnaB

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI, tyrR::P_trc-aroG^S180F, trpR::P_trc-serA^H344A,N364A, trpE::P_trc-trpE^S40F, ΔpykF, tktA::P_trc-tktA, ΔptsH, yjiV::P_J23105-UTRWP1-galP, ylbE::P_J23114-UTRMK3-glk, PrpsT p1-ppc, Prrnc p1-gltA | ycjv::P_trc-aroEK | mbhA::P_trc-BS.glnA^L159I,E304A | yeeP::P_serB-serB, P_serC-serC | ΔTnaB

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
tnaB	Escherichia coli	Silencing

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-tryptophan	Titer	53.65 g/L		Glucose	42 h	Fed-Batch	[183]
L-tryptophan	Substrate yield	0.238 g/g of sustrate		Glucose	42 h	Fed-Batch	[183]
L-tryptophan	Titer	14.12 g/L	6.7 OD₆₀₀^*	Glucose		Flask	[183]
L-tryptophan	Substrate yield	0.155 g/g of sustrate ^*	6.7 OD₆₀₀^*	Glucose		Flask	[183]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Liu S, Wang BB, Xu JZ, Zhang WG. Engineering of Shikimate Pathway and Terminal Branch for Efficient Production of L-Tryptophan in Escherichia coli. Int J Mol Sci. 2023 Jul 24;24(14):11866.