Escherichia coli TH28C (pBRThrABCR3)

Ancestors

Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI | thrAfbr | lysCfbr | Pthr::Ptac | ΔlysA | ΔmetA | ilvAC290T | Δtdh | iclR::CmR | Pppc::Ptrc | tdcC::CmR | Pacs::CmR-Pprc | Plasmid pBRThrABCR3 added

Bars (|) indicate differences between strains.


Plasmids

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
L-threonine Substrate yield 0.294 g/g of sustrate 35.6 g/L 50 h Fed-Batch [190]
L-threonine Titer 82.4 g/L 35.6 g/L 50 h Fed-Batch [190]
acetate Titer 2.35 g/L 35.6 g/L 50 h Fed-Batch [190]
L-threonine Substrate yield 0.273 g/g of sustrate 33.35 g/L 52 h Fed-Batch [190]
L-threonine Substrate yield 0.268 g/g of sustrate 32.97 g/L 52 h Fed-Batch [190]
L-threonine Titer 76.5 g/L 33.35 g/L 52 h Fed-Batch [190]
acetate Titer 2.41 g/L 33.35 g/L 52 h Fed-Batch [190]
L-threonine Titer 75.1 g/L 32.97 g/L 52 h Fed-Batch [190]
acetate Titer 2.16 g/L 32.97 g/L 52 h Fed-Batch [190]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References