Escherichia coli KW018

Ancestors

Derived strains

Genotype with respect to parental

M1-93::glk


Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | Plasmid PH5a1 added | ΔtrpR ΔtrpL | ΔpykF | ΔptsH | M1-37::galP | M1-93::glk

Bars (|) indicate differences between strains.


Plasmids

Strain derived from a randomly mutated strain.


Modifications
Gene Organism Modification
glk
Overexpression

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
L-tryptophan Titer 2.3 g/L Flask [135]
L-tryptophan Titer 37.0 g/L 48 h Fed-Batch [135]
L-tryptophan Volumetric productivity 1.5 g/L/h 28 h Fed-Batch [135]
L-tryptophan Substrate yield 0.17 g/g of sustrate 28 h Fed-Batch [135]
acetate Titer 3.5 g/L 46 h Fed-Batch [135]
L-tryptophan Substrate yield 0.11 g/g of sustrate Flask [135]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References