Escherichia coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)

Ancestors

Genotype with respect to parental

λ(DE3)


Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔsdaA | ΔsdaB ΔtdcG | ΔglyA | λ(DE3) | λ(DE3) | Plasmid pACTCDuet-1-serB added | Plasmid pCDF-Duet-serAmut-serC added

Bars (|) indicate differences between strains.


Plasmids

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
L-serine Titer 0.89 g/L Flask [144]
L-serine Volumetric productivity 2.73 g/L/h Flask [144]
L-serine Titer 8.3 g/L Fed-Batch [144]
L-serine Volumetric productivity 0.18 g/L/h Fed-Batch [144]
L-serine Substrate yield 0.12 g/g of sustrate Fed-Batch [144]
L-serine Titer 3.7 g/L 52 h Fed-Batch [192]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References