Escherichia coli Q3 (DE3) (pCDF-Duet-serAmut-serC, pACYCDuet-1-serB-eamA-c-His)

Ancestors

Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔsdaA | ΔsdaB ΔtdcG | ΔglyA | rhtA mntS | λ(DE3) | Plasmid pCDF-Duet-serAmut-serC added | Plasmid pACYCDuet-1-serB-eamA-c-His added

Bars (|) indicate differences between strains.


Plasmids

Strain derived from a randomly mutated strain.


Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
L-serine Titer 11.73 g/L Fed-Batch [144]
L-serine Volumetric productivity 0.24 g/L/h Fed-Batch [144]
L-serine Substrate yield 0.15 g/g of sustrate Fed-Batch [144]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References