Escherichia coli ALE-4-2 (DE3) ( (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)

Ancestors

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔsdaA | ΔsdaB ΔtdcG | ΔglyA | λ(DE3) | Plasmid pACTCDuet-1-serB added | Plasmid pCDF-Duet-serAmut-serC added

Bars (|) indicate differences between strains.

Plasmids

• pCDF-Duet-serAmut-serC (serA^mut serC)

  pACTCDuet-1-serB (serB)

Strain derived from a randomly mutated strain.

References

• Hemanshu Mundhada, Jose M. Seoane, Konstantin Schneider, Anna Koza, Hanne B. Christensen, Tobias Klein, Patrick V. Phaneuf, Markus Herrgard, Adam M. Feist & Alex T. Nielsen (2016). Increased production of L-serine in Escherichia coli through Adaptive Laboratory Evolution. Metabolic Engineering.