Escherichia coli TS-10
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | Plasmid PH5a1 added | ΔglnA::BSglnA | ΔackA::J23119-icd ΔpoxB::J23119-gdhA | ΔatpI::Ptrc::prs | serAmut | thrAmut | ΔlacZ::J23119::sthA-pntAB | Plasmid PH5a1 lost | Plasmid pXlltS addedBars (|) indicate differences between strains.
Plasmids
-
pXlltS (aroGfbr trpEDCBA sthA)
Strain derived from a randomly mutated strain.
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-tryptophan | Titer | 1.71 g/L | 48 h | Flask | [136] | ||
| acetate | Titer | 0.076 g/L | 48 h | Flask | [136] | ||
| L-tryptophan | Substrate yield | 6.7 % | 48 h | Flask | [136] | ||
| 2-oxoglutarate | Titer | 1.104 g/L | 48 h | Flask | [136] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.