Escherichia coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔmetJ | ΔmetI | ΔlysA | Plasmid pTrcA*H addedBars (|) indicate differences between strains.
Plasmids
-
pTrcA*H (Ptrc-metAfbr(A881G)-yjeH)
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-methionine | Titer | 4.08 g/L | Flask | [204] | |||
| L-methionine | Substrate yield | 0.19 g/g of sustrate | Batch | [204] | |||
| L-methionine | Titer | 9.75 g/L | 14.22 g/L | Fed-Batch | [204] | ||
| L-methionine | Substrate yield | 14.0 % | 14.22 g/L | Fed-Batch | [204] | ||
| L-methionine | Volumetric productivity | 0.2 g/L/h | 14.22 g/L | Fed-Batch | [204] | ||
| L-aspartate | Titer | 1.47 g/L | 14.22 g/L | Fed-Batch | [204] | ||
| L-glutamate | Titer | 1.55 g/L | 14.22 g/L | Fed-Batch | [204] | ||
| acetate | Titer | 1.78 g/L | 14.22 g/L | Fed-Batch | [204] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.