Escherichia coli TP1

Ancestors

Genotype with respect to wild type

F⁺ (λ) | aroH367, tyrR366, tna-2, lacY5, aroF394^fbr, malT384, pheA101^fbr, pheO352, aroG397^fbr | ΔpheLA ΔtyrA | Plasmid pSA-trpDBCA added | Plasmid pCS-trpE^fbrtrpG-serA*-prsA-A95T added

Bars (|) indicate differences between strains.

Plasmids

pCS-trpE^fbrtrpG-serA*-prsA-A95T (P_LlacO1-trpE^fbrtrpG, P_LlacO1-serA*-prsA-A95T)

pSA-trpDBCA (trpDBCA)

Production

Metabolites	Production type	Production	Carbon source	Time	Scale	Ref.
L-tryptophan	Titer	0.73 g/L	Glycerol	60 h	Flask	[205]
L-tryptophan	Substrate yield	0.038 g/g of sustrate	Glycerol	60	Flask	[205]
anthranilate	Titer	0.0 g/L	Glycerol	60 h	Flask	[205]
L-tryptophan	Titer	0.82 g/L	Xylose	36 h	Flask	[205]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Jian Wang, Ruihua Zhang, Yan Zhang, Yaping Yang, Yuheng Lin & Yajun Yan (2019). Developing a pyruvate-driven metabolic scenario for growth-coupled microbial production. Metabolic Engineering.