Escherichia coli TP3
Ancestors
Genotype with respect to wild type
F+ (λ) | aroH367, tyrR366, tna-2, lacY5, aroF394fbr, malT384, pheA101fbr, pheO352, aroG397fbr | ΔpheLA ΔtyrA | ΔpykA ΔpykF | ΔgldA | ΔmaeB | ΔtnaA ΔtrpR | Plasmid F' added | Plasmid pSA-trpDBCA added | Plasmid pCS-trpEfbrtrpG-serA*-prsA-A95T addedBars (|) indicate differences between strains.
Plasmids
-
F' (traD36 proAB lacIq ZΔM15)
pCS-trpEfbrtrpG-serA*-prsA-A95T (PLlacO1-trpEfbrtrpG, PLlacO1-serA*-prsA-A95T)
pSA-trpDBCA (trpDBCA)
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-tryptophan | Substrate yield | 0.168 g/g of sustrate | 60 h | Flask | [205] | ||
| L-tryptophan | Titer | 0.97 g/L | 36 h | Flask | [205] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.