Escherichia coli B0016-060B

Ancestors

All strains

Escherichia coli B0016

Escherichia coli B0016-050

Escherichia coli B0016-060B

Genotype with respect to parental

ΔdadX::FRT

Genotype with respect to wild type

Δack-pta ΔpflB ΔadhE ΔfrdA ΔldhA | ΔdadX::FRT

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
dadX	Escherichia coli	Silencing

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-alanine	Titer	0.02 g/L		Glucose		Flask	[140]
L-alanine	Substrate yield	0.002 g/L		Glucose		Flask	[140]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Zhou, L., Deng, C., Cui, WJ., Liu, ZM., Zhou, ZM. (2016). Efficient L-Alanine Production by a Thermo-Regulated Switch in Escherichia coli . Appl Biochem Biotechnol.

Li, Jinyang; Shen, Jiawen; Ye, Wuyue; Tang, Xinyan; Wang, Zhiyu; Geng, Muyun; Liu, Yunye; Chen, Xianzhong & Zhou, Li. Dynamically metabolic engineering overflow metabolism for efficient production of l-alanine in Escherichia coli. Bioresource Technology. 2025, 428, 132446.