Escherichia coli CWF811
Ancestors
Derived strains
Genotype with respect to parental
ΔlacI
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔiclR | ΔfumC | ΔfumA | ΔfumB | ΔarcA | Plasmid pTac15kppc added | ΔptsG | ΔaspA | ΔlacIBars (|) indicate differences between strains.
Plasmids
-
pTac15kppc (Ptac-ppc)
Modifications
| Gene | Organism | Modification |
|---|---|---|
|
Silencing
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| fumarate | Titer | 6.25 g/L | 24 h | Flask | [210] | ||
| fumarate | Substrate yield | 0.417 g/g of sustrate | 24 h | Flask | [210] | ||
| fumarate | Titer | 8.23 g/L | 27 h | Batch | [210] | ||
| fumarate | Substrate yield | 0.415 g/g of sustrate | 27 h | Batch | [210] | ||
| fumarate | Volumetric productivity | 0.305 g/L/h | 27 h | Batch | [210] | ||
| fumarate | Biomass-specific productivity | 0.201 g/g DCW/h | 27 h | Batch | [210] | ||
| acetate | Titer | 2.0 g/L | 27 h | Batch | [210] | ||
| acetate | Substrate yield | 0.1 g/g of sustrate | 27 h | Batch | [210] | ||
| fumarate | Titer | 26.2 g/L | 104 h | Fed-Batch | [210] | ||
| fumarate | Substrate yield | 0.361 g/g of sustrate | 104 h | Fed-Batch | [210] | ||
| fumarate | Volumetric productivity | 0.252 g/L/h | 104 h | Fed-Batch | [210] | ||
| acetate | Titer | 9.55 g/L | 104 h | Fed-Batch | [210] | ||
| acetate | Substrate yield | 0.132 g/g of sustrate | 104 h | Fed-Batch | [210] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.