Escherichia coli CWF812

Ancestors

Genotype with respect to parental

PgalP::Ptrc


Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔiclR | ΔfumC | ΔfumA | ΔfumB | ΔarcA | Plasmid pTac15kppc added | ΔptsG | ΔaspA | ΔlacI | PgalP::Ptrc

Bars (|) indicate differences between strains.


Plasmids

Modifications
Gene Organism Modification
Overexpression

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
fumarate Titer 7.85 g/L 20 h Batch [210]
fumarate Substrate yield 0.394 g/g of sustrate 20 h Batch [210]
fumarate Volumetric productivity 0.393 g/L/h 20 h Batch [210]
fumarate Biomass-specific productivity 0.238 g/g DCW/h 20 h Batch [210]
acetate Titer 2.0 g/L 20 h Batch [210]
acetate Substrate yield 0.1 g/g of sustrate 20 h Batch [210]
fumarate Titer 28.2 g/L 63 h Fed-Batch [210]
fumarate Substrate yield 0.389 g/g of sustrate 63 h Fed-Batch [210]
fumarate Volumetric productivity 0.448 g/L/h 63 h Fed-Batch [210]
acetate Titer 5.24 g/L 63 h Fed-Batch [210]
acetate Substrate yield 0.072 g/g of sustrate 63 h Fed-Batch [210]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References