Escherichia coli PM-14

Ancestors

Derived strains

• Escherichia coli M1

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | Plasmid pET-28aA added

Bars (|) indicate differences between strains.

Plasmids

• pET-28aA (kan^R-A (rare codon usage))

Strain generated through random mutagenesis.

References

• Long M, Xu M, Ma Z, Pan X, You J, Hu M, Shao Y, Yang T, Zhang X, Rao Z. Significantly enhancing production of trans-4-hydroxy-l-proline by integrated system engineering in Escherichia coli. Sci Adv. 2020 May 22;6(21):eaba2383.