Escherichia coli M6
Ancestors
Derived strains
Genotype with respect to parental
proBfbr (D107N/E143A)
Genotype with respect to wild type
F− dcm gal ompT hsdS(rB- mB-) | F− ompT hsdSB (rB−mB−) gal dcm λ(DE3) | Plasmid pET-28aA added | Plasmid pET-28aA lost | ΔputA | ΔputP | ΔproP | ΔaceA | proBfbr (D107N/E143A)Bars (|) indicate differences between strains.
Strain derived from a randomly mutated strain.
Modifications
| Gene | Organism | Modification |
|---|---|---|
|
Feedback insensitive
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-proline | Titer | 37.5 g/L | Fed-Batch | [212] | |||
| L-proline | Volumetric productivity | 0.938 g/L/h | Fed-Batch | [212] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.