Escherichia coli M5/proBE143A

Ancestors
Genotype with respect to parental

proBfbr(E143A)


Genotype with respect to wild type
F dcm gal ompT hsdS(rB- mB-) | F ompT hsdSB (rBmB) gal dcm λ(DE3) | Plasmid pET-28aA added | Plasmid pET-28aA lost | ΔputA | ΔputP | ΔproP | ΔaceA | proBfbr(E143A)

Bars (|) indicate differences between strains.


Strain derived from a randomly mutated strain.


Modifications
Gene Organism Modification
Feedback insensitive

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
L-proline Titer 33.8 g/L Fed-Batch [212]
L-proline Volumetric productivity 0.845 g/L/h Fed-Batch [212]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References