Escherichia coli M8-2

Ancestors

Derived strains

• Escherichia coli HYP2
• Escherichia coli HYP2M

Genotype with respect to parental

P_esaS:: P_sucA

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | Plasmid pET-28aA added | Plasmid pET-28aA lost | ΔputA | ΔputP | ΔproP | ΔaceA | proB^fbr (D107N/E143A) | esaR(I70V)::proP | P_bs2-esaI::putA | P_esaS:: P_sucA

Bars (|) indicate differences between strains.

Strain derived from a randomly mutated strain.

References

• Long M, Xu M, Ma Z, Pan X, You J, Hu M, Shao Y, Yang T, Zhang X, Rao Z. Significantly enhancing production of trans-4-hydroxy-l-proline by integrated system engineering in Escherichia coli. Sci Adv. 2020 May 22;6(21):eaba2383.