Escherichia coli HYP3

Ancestors

All strains

Escherichia coli B

Escherichia coli BL21

Escherichia coli BL21(DE3)

Escherichia coli PM-14

Escherichia coli M1

Escherichia coli M2

Escherichia coli M3

Escherichia coli M4

Escherichia coli M5

Escherichia coli M6

Escherichia coli M6R

Escherichia coli M7-3

Escherichia coli M8-3

Escherichia coli HYP3

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | Plasmid pET-28aA added | Plasmid pET-28aA lost | ΔputA | ΔputP | ΔproP | ΔaceA | proB^fbr (D107N/E143A) | esaR(I70V)::proP | P_bs3-esaI::putA | P_esaS::P_sucA | Plasmid pDXW-10-Datp4h added

Bars (|) indicate differences between strains.

Plasmids

pDXW-10-Datp4h (Datp4h^opt)

Strain derived from a randomly mutated strain.

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
trans-4-hydroxy-L-proline	Titer	15.4 g/L		Glucose		Fed-Batch	[212]
trans-4-hydroxy-L-proline	Volumetric productivity	0.06 g/L/h		Glucose		Fed-Batch	[212]
trans-4-hydroxy-L-proline	Substrate yield	0.37 g/g of sustrate		Glucose		Fed-Batch	[212]
L-proline	Titer	8.9 g/L		Glucose		Fed-Batch	[212]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Long M, Xu M, Ma Z, Pan X, You J, Hu M, Shao Y, Yang T, Zhang X, Rao Z. Significantly enhancing production of trans-4-hydroxy-l-proline by integrated system engineering in Escherichia coli. Sci Adv. 2020 May 22;6(21):eaba2383.