Escherichia coli SA5/pTH-aroG*-ppsA-tktA

Ancestors

Genotype with respect to wild type
ΔaroL::dif | ΔaroK::dif | ΔptsG::dif | ΔydiB::dif | ΔackA-pta::dif | Plasmid pTH-aroGfbr-ppsA-tktA added

Bars (|) indicate differences between strains.


Plasmids

Strain derived from a randomly mutated strain.


Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
shikimate Titer 1207.0 mg/L 27 h Flask [218]
shikimate Substrate yield 85.1 mg/g of sustrate 27 h Flask [218]
L-quinate Titer 0.68 g/L 50 h Fed-Batch [218]
shikimate Titer 14.6 g/L 50 h Fed-Batch [218]
shikimate Substrate yield 0.2925 g/g of sustrate 50 h Fed-Batch [218]
3-dehydroquinate Titer 0.87 g/L 50 h Fed-Batch [218]
shikimate Volumetric productivity 0.047 g/L/h 27 h Flask [218]
shikimate Volumetric productivity 0.26 g/L/h 50 h Fed-Batch [218]
L-quinate Substrate yield 0.0136 g/g of sustrate 50 h Fed-Batch [218]
L-quinate Substrate yield 0.0059 g/g of sustrate 27 h Flask [218]
3-dehydroquinate Substrate yield 0.0288 g/g of sustrate 27 h Flask [218]
3-dehydroquinate Substrate yield 0.0174 g/g of sustrate 50 h Fed-Batch [218]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References