Escherichia coli 7107-87
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | F- λ- rph-1 IN(rrnD-rrnE)1 Thy | Δnag::tetR manXYZ8 DE3 | ΔgalK::T7lac–glmS*54 | Plasmid pET24d(+)/T7lac-ScGNA1 addedBars (|) indicate differences between strains.
Plasmids
-
pET24d(+)/T7lac-ScGNA1 (PT7lac-ScGNA1)
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| N-acetyl-β-D-glucosamine | Titer | 11.7 g/L | 7.8 OD600 | 23 h | Flask | [220] | |
| acetate | Titer | 0.0 g/L | 7.8 OD600 | 23 h | Flask | [220] | |
| N-acetyl-β-D-glucosamine | Titer | 22.4 g/L | 48 h | Flask | [220] | ||
| N-acetyl-β-D-glucosamine | Titer | 50.0 g/L | 70 h | Fed-Batch | [220] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.
References
- Ming-De Deng, David K. Severson, Alan D. Grund, Sarah L. Wassink, Richard P. Burlingame, Alan Berry, Jeffrey A. Running, Candice A. Kunesh, Linsheng Song, Thomas A. Jerrell & Reinhardt A. Rosson (2005). Metabolic engineering of Escherichia coli for industrial production of glucosamine and N-acetylglucosamine. Metabolic Engineering.