Escherichia coli 050T4/pCTSDT

Ancestors

Genotype with respect to wild type
Δack-pta ΔpflB ΔadhE ΔfrdA ΔldhA | ilvE::T7RNAP | ΔleuA | PpntA::PT7 | Plasmid pCTSDT added

Bars (|) indicate differences between strains.


Plasmids

  • pCTSDT (PT7-ilvCEc-TT7-PT7-alsSBs-PT7-ilvDEc-TT7)


Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
3-methyl-2-oxobutanoate Titer 46.4 g/L Fed-Batch [221]
3-methyl-2-oxobutanoate Volumetric productivity 1.78 g/L/h Fed-Batch [221]
3-methyl-2-oxobutanoate Biomass-specific productivity 0.112 g/g DCW/h Fed-Batch [221]
3-methyl-2-oxobutanoate Substrate yield 0.644 mol/mol of substrate Fed-Batch [221]
isobutanol Substrate yield 0.284 mol/mol of substrate Fed-Batch [221]
pyruvate Substrate yield 0.016 mol/mol of substrate Fed-Batch [221]
succinate Substrate yield 0.119 mol/mol of substrate Fed-Batch [221]
acetate Substrate yield 0.075 mol/mol of substrate Fed-Batch [221]
3-methyl-2-oxobutanoate Titer 10.83 g/L * 6.53 OD600* Flask [221]
3-methyl-2-oxobutanoate Titer 15.2 g/L 36 h Flask [221]
3-methyl-2-oxobutanoate Titer 19.2 g/L Flask [221]
3-methyl-2-oxobutanoate Substrate yield 0.797 mol/mol of substrate Flask [221]
isobutanol Titer 13.1 g/L Fed-Batch [221]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References