Escherichia coli 050TY/pCTSDTQ487S-RBS55

Ancestors

Genotype with respect to wild type
Δack-pta ΔpflB ΔadhE ΔfrdA ΔldhA | ilvE::T7RNAP | ΔleuA | PpntA::PT7 | aceF-TagDAS+4 | Plasmid pCTSDTQ487S-RBS55 added

Bars (|) indicate differences between strains.


Plasmids

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
3-methyl-2-oxobutanoate Titer 55.8 g/L Fed-Batch [221]
3-methyl-2-oxobutanoate Volumetric productivity 2.14 g/L/h Fed-Batch [221]
3-methyl-2-oxobutanoate Biomass-specific productivity 0.162 g/g DCW/h Fed-Batch [221]
3-methyl-2-oxobutanoate Substrate yield 0.852 mol/mol of substrate Fed-Batch [221]
isobutanol Substrate yield 0.036 mol/mol of substrate Fed-Batch [221]
pyruvate Substrate yield 0.022 mol/mol of substrate Fed-Batch [221]
succinate Substrate yield 0.04 mol/mol of substrate Fed-Batch [221]
acetate Substrate yield 0.134 mol/mol of substrate Fed-Batch [221]
3-methyl-2-oxobutanoate Titer 22.0 g/L Flask [221]
3-methyl-2-oxobutanoate Substrate yield 0.94 mol/mol of substrate Flask [221]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References