Escherichia coli 050T3/pCTSDT+pACYC-pntAB
Ancestors
Genotype with respect to wild type
Δack-pta ΔpflB ΔadhE ΔfrdA ΔldhA | ilvE::T7RNAP | ΔleuA | Plasmid pACYC-pntAB added | Plasmid pCTSDT addedBars (|) indicate differences between strains.
Plasmids
-
pCTSDT (PT7-ilvCEc-TT7-PT7-alsSBs-PT7-ilvDEc-TT7)
pACYC-pntAB (pntAB, pntAB)
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| 3-methyl-2-oxobutanoate | Titer | 9.35 g/L * | 9.16 OD600* | Flask | [221] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.