Escherichia coli W3110 trpD9923/pJLBaroGfbrtktA
Ancestors
Derived strains
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | trpD9923 | Plasmid pJLBaroGfbrtktA addedBars (|) indicate differences between strains.
Plasmids
-
pJLBaroGfbrtktA (lacIq, PlacUV5-aroGfbr, PtktA-tktA)
Strain derived from a randomly mutated strain.
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| anthranilate | Titer | 14.0 g/L | 18.54 g/L | 34 h | Fed-Batch | [151] | |
| anthranilate | Titer | 0.75 g/L | 0.93 g/L | Flask | [151] | ||
| anthranilate | Substrate yield | 0.2 g/g of sustrate | 18.54 g/L | 34 h | Fed-Batch | [151] | |
| acetate | Titer | 0.5 g/L | 18.54 g/L | 34 | Fed-Batch | [151] | |
| anthranilate | Biomass-specific productivity | 0.02 g/g DCW/h | 18.54 g/L | 34 h | Fed-Batch | [236] | |
| anthranilate | Titer | 8.18 g/L | 7.66 g/L | Fed-Batch | [236] | ||
| anthranilate | Substrate yield | 0.17 g/g of sustrate | 7.66 g/L | Fed-Batch | [236] | ||
| anthranilate | Biomass-specific productivity | 0.02 g/g DCW/h | 7.66 g/L | Fed-Batch | [236] | ||
| anthranilate | Titer | 8.61 g/L | 7.51 g/L | Fed-Batch | [236] | ||
| anthranilate | Substrate yield | 0.18 g/g of sustrate | 7.51 g/L | Fed-Batch | [236] | ||
| anthranilate | Biomass-specific productivity | 0.02 g/g DCW/h | 7.51 g/L | Fed-Batch | [236] | ||
| anthranilate | Biomass-specific productivity | 0.01 g/g DCW/h | 7.37 g/L | Fed-Batch | [236] | ||
| anthranilate | Substrate yield | 0.11 g/g of sustrate | 7.37 g/L | Fed-Batch | [236] | ||
| anthranilate | Titer | 6.28 g/L | 7.37 g/L | Fed-Batch | [236] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.
References
- Víctor E Balderas-Hernández, Andrea Sabido-Ramos, Patricia Silva, Natividad Cabrera-Valladares, Georgina Hernández-Chávez, José L Báez-Viveros, Alfredo Martínez, Francisco Bolívar & Guillermo Gosset (2009). Metabolic engineering for improving anthranilate synthesis from glucose in Escherichia coli. Microbial Cell Factories.