Escherichia coli XB00

Ancestors

Genotype with respect to wild type

F⁺ (λ) | IacI^q rrnB_T14 ∆lacZ_WJ16 hsdR514 ∆araBAD_AH33 ∆rhaBAD_LD78 | F׳ proAB lacI^qZΔM15 Tn10 (Tet^r) | Plasmid pLB1a-EC added | Plasmid pXB1k-BD added

Bars (|) indicate differences between strains.

Plasmids

pLB1a-EC (P_araBAD-aspC)

pXB1k-BD (P_araBAD-panD_Bs)

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-alanine	Titer	1.34 mM ^*		Glucose		Flask	[223]
β-alanine	Titer	1.38 mM ^*		Glucose		Flask	[223]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Xiaoyu Piao, Lei Wang, Baixue Lin, Hao Chen, Weifeng Liu & Yong Tao (2019). Metabolic engineering of Escherichia coli for production of L-aspartate and its derivative β-alanine with high stoichiometric yield. Metabolic Engineering.