Escherichia coli XBR21

Ancestors

All strains

Escherichia coli K12

Escherichia coli BW25113

Escherichia coli BW25113/F'

Escherichia coli XY01

Escherichia coli XY02

Escherichia coli XY04

Escherichia coli XY11

Escherichia coli XY21

Escherichia coli XBR21

Genotype with respect to wild type

F⁺ (λ) | IacI^q rrnB_T14 ∆lacZ_WJ16 hsdR514 ∆araBAD_AH33 ∆rhaBAD_LD78 | F׳ proAB lacI^qZΔM15 Tn10 (Tet^r) | ΔompT::P_tac-ppc_Cg | ΔpykA ΔpykF | Δmdh | ΔaspA | ΔgalR, ptsG::Ptac-glk | Plasmid pLB1a-CR added | Plasmid pXB1k-BD added

Bars (|) indicate differences between strains.

Plasmids

pXB1k-BD (P_araBAD-panD_Bs)

pLB1a-CR (P_araBAD-aspC_Ec rocG_Bs)

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
β-alanine	Titer	65.44 mM		Glucose		Flask	[223]
β-alanine	Substrate yield	1.3 mol/mol of substrate		Glucose		Flask	[223]
acetate	Titer	6.57 mM ^*		Glucose		Flask	[223]
(R)-lactate	Titer	2.4 mM ^*		Glucose		Flask	[223]
formate	Titer	0.0 mM ^*		Glucose		Flask	[223]
succinate	Titer	4.49 mM ^*		Glucose		Flask	[223]
β-alanine	Titer	54.6 mM ^*		Glucose		Flask	[223]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Xiaoyu Piao, Lei Wang, Baixue Lin, Hao Chen, Weifeng Liu & Yong Tao (2019). Metabolic engineering of Escherichia coli for production of L-aspartate and its derivative β-alanine with high stoichiometric yield. Metabolic Engineering.