Escherichia coli FB-04(Δpta)

Ancestors

Genotype with respect to parental

Δpta::kan-sacB


Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔtrpR | ΔtnaA | ΔpheA | ΔtyrA::FRT | Δpta::kan-sacB | Plasmid pSV03 added

Bars (|) indicate differences between strains.


Plasmids

  • pSV03 (p15A ori, aroFfbr trpEfbrD)


Modifications
Gene Organism Modification
pta
Silencing
Overexpression

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
L-tryptophan Titer 1.4 g/L 6.2 OD600 48 h Flask [146]
L-tryptophan Substrate yield 0.04 g/g of sustrate 6.2 OD600 48 h Flask [146]
L-tryptophan Volumetric productivity 0.029 g/L/h 6.2 OD600 48 h Flask [146]
acetate Titer 3.6 g/L 6.2 OD600 48 h Flask [146]
L-tryptophan Titer 21.8 g/L 52.5 OD600 54 h Fed-Batch [146]
L-tryptophan Substrate yield 0.08 g/g of sustrate 52.5 OD600 54 h Fed-Batch [146]
L-tryptophan Volumetric productivity 0.4 g/L/h 52.5 OD600 54 h Fed-Batch [146]
acetate Titer 0.5 g/L 52.5 OD600 54 h Fed-Batch [146]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References