Escherichia coli XZ113

Ancestors

Derived strains

Escherichia coli XZ115

Genotype with respect to parental

Metabolic evolution in NBS medium with 5% glucose

Genotype with respect to wild type

ΔpflB ΔfrdABCD ΔadhE ΔackA | ldhA::FRT-tet-FRT::alaD_Gs | Δtet | Metabolic evolution in NBS medium with 2% glucose | Metabolic evolution in NBS medium with 5% glucose

Bars (|) indicate differences between strains.

Strain generated through random mutagenesis.

Production

Metabolites	Production type	Production	Carbon source	Time	Scale	Ref.
L-alanine	Titer	483.0 mM	Glucose	48 h	Batch	[208]
L-alanine	Substrate yield	86.0 %	Glucose	48 h	Batch	[208]
(R)-lactate	Titer	4.0 mM	Glucose	48 h	Batch	[208]
acetate	Titer	7.0 mM	Glucose	48 h	Batch	[208]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Xueli Zhang, Kaemwich Jantama, J. C. Moore, K. T. Shanmugam & L. O. Ingram (2007). Production of l-alanine by metabolically engineered Escherichia coli. Applied Microbiology and Biotechnology.