Escherichia coli XZ129

Ancestors

All strains

Escherichia coli SZ194

Escherichia coli XZ105

Escherichia coli XZ111

Escherichia coli XZ112

Escherichia coli XZ113

Escherichia coli XZ115

Escherichia coli XZ121

Escherichia coli XZ123

Escherichia coli XZ126

Escherichia coli XZ129

Derived strains

Escherichia coli XZ130

Genotype with respect to parental

Metabolic evolution in NBS medium with 8% glucose

Genotype with respect to wild type

ΔpflB ΔfrdABCD ΔadhE ΔackA | ldhA::FRT-tet-FRT::alaD_Gs | Δtet | Metabolic evolution in NBS medium with 2% glucose | Metabolic evolution in NBS medium with 5% glucose | Metabolic evolution in NBS medium with 8% glucose | ΔmgsA | Metabolic evolution in NBS medium with 8% glucose | ΔdadX | Metabolic evolution in NBS medium with 8% glucose

Bars (|) indicate differences between strains.

Strain generated through random mutagenesis.

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-alanine	Titer	833.0 mM	2.03 g/L	Glucose	42 h	Batch	[208]
L-alanine	Substrate yield	0.93 g/g of sustrate	2.03 g/L	Glucose	42 h	Batch	[208]
succinate	Titer	2.0 mM	2.03 g/L	Glucose	42 h	Batch	[208]
acetate	Titer	8.0 mM	2.03 g/L	Glucose	42 h	Batch	[208]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Xueli Zhang, Kaemwich Jantama, J. C. Moore, K. T. Shanmugam & L. O. Ingram (2007). Production of l-alanine by metabolically engineered Escherichia coli. Applied Microbiology and Biotechnology.