Escherichia coli XZ132
Ancestors
Genotype with respect to parental
Metabolic evolution in AM1 medium with 12% glucose
Genotype with respect to wild type
ΔpflB ΔfrdABCD ΔadhE ΔackA | ldhA::FRT-tet-FRT::alaDGs | Δtet | Metabolic evolution in NBS medium with 2% glucose | Metabolic evolution in NBS medium with 5% glucose | Metabolic evolution in NBS medium with 8% glucose | ΔmgsA | Metabolic evolution in NBS medium with 8% glucose | ΔdadX | Metabolic evolution in NBS medium with 8% glucose | Metabolic evolution in AM1 medium with 8% glucose | Metabolic evolution in AM1 medium with 10% glucose | Metabolic evolution in AM1 medium with 12% glucoseBars (|) indicate differences between strains.
Strain generated through random mutagenesis.
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-alanine | Titer | 1279.0 mM | 2.03 g/L | 48 h | Batch | [208] | |
| L-alanine | Substrate yield | 0.95 g/g of sustrate | 2.03 g/L | 48 h | Batch | [208] | |
| succinate | Titer | 2.0 mM | 2.03 g/L | 48 h | Batch | [208] | |
| acetate | Titer | 7.0 mM | 2.03 g/L | 48 h | Batch | [208] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.