Escherichia coli EYX-2

Ancestors

Derived strains

Genotype with respect to wild type
F+ (λ) | IacIq rrnBT14 ∆lacZWJ16 hsdR514 ∆araBADAH33 ∆rhaBADLD78 | ΔaceB | ΔglcB::WAK cassette (ycdW, aceA-aceK)

Bars (|) indicate differences between strains.


Strain generated through random mutagenesis.


Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
glycolate Substrate yield 0.48 g/g of sustrate Flask [214]
glycolate Titer 56.44 g/L 19.26 OD600 120 h Fed-Batch [225]
glycolate Substrate yield 0.52 g/g of sustrate 19.26 OD600 120 h Fed-Batch [225]
glycolate Substrate yield 0.33 g/g of sustrate 2.11 OD600* Flask [225]
glycolate Substrate yield 0.48 g/g of sustrate 24 h Batch [225]
glycolate Titer 2.66 g/L 2.11 OD600* Flask [225]
glycolate Titer 8.96 g/L 24 h Batch [225]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References