Escherichia coli FB-04(ΔackA)

Ancestors

Genotype with respect to parental

ΔackA::kan-sacB


Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔtrpR | ΔtnaA | ΔpheA | ΔtyrA::FRT | ΔackA::kan-sacB | Plasmid pSV03 added

Bars (|) indicate differences between strains.


Plasmids

  • pSV03 (p15A ori, aroFfbr trpEfbrD)


Modifications
Gene Organism Modification
Silencing
Overexpression

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
L-tryptophan Titer 1.3 g/L 7.7 OD600 48 h Flask [146]
L-tryptophan Substrate yield 0.032 g/g of sustrate 7.7 OD600 48 h Flask [146]
L-tryptophan Volumetric productivity 0.74 g/L/h 7.7 OD600 48 h Flask [146]
acetate Titer 4.9 g/L 7.7 OD600 48 h Flask [146]
L-tryptophan Titer 40.2 g/L 76.8 OD600 54 h Fed-Batch [146]
L-tryptophan Substrate yield 0.12 g/g of sustrate 76.8 OD600 54 h Fed-Batch [146]
L-tryptophan Volumetric productivity 0.74 g/L/h 76.8 OD600 54 h Fed-Batch [146]
acetate Titer 1.5 g/L 76.8 OD600 54 h Fed-Batch [146]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References