Escherichia coli ILE01 (pTacilvAIH, pBRthrABCygaZH)
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI | thrAfbr | lysCfbr | Pthr::Ptac | ΔlysA | ΔmetA | ilvAC290T | Δtdh | iclR::CmR | Pppc::Ptrc | ΔCmR | ilvAC1339T, G1341T, C1351G, T1352C | Plasmid pBRthrABCygaZH added | Plasmid pTacilvAIH addedBars (|) indicate differences between strains.
Plasmids
-
pTacilvAIH (ilvAIH)
pBRthrABCygaZH (thrAfbrBC ygaZH)
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-isoleucine | Titer | 1.11 g/L | Flask | [228] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.