Escherichia coli PA109
Ancestors
Genotype with respect to parental
Replacing the start codon of gltA from ATG to GTG
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Grows vigorously in the presence of β-alanine | ilvG :: PM1-93-alsScgl, panBC :: PM1-93-panBC | Δglk | ΔavtA | Replacing the start codon of ilvE from ATG to GTG | ΔldhA | ΔpoxB | Replacing the start codon of gltA from ATG to GTGBars (|) indicate differences between strains.
Strain derived from a randomly mutated strain.
Modifications
| Gene | Organism | Modification |
|---|---|---|
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Attenuation
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| (R)-pantothenate | Titer | 0.94 g/L | 48 h | Flask | [233] | ||
| (R)-pantothenate | Biomass-specific titer | 0.639 g/L/OD600 * | 60 h * | Flask | [233] | ||
| (R)-pantothenate | Biomass-specific titer | 0.033 g/L/OD600 * | 60 h * | Flask | [233] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.