Escherichia coli PA111 pEc

Ancestors

Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Grows vigorously in the presence of β-alanine | ilvG :: PM1-93-alsScgl, panBC :: PM1-93-panBC | Δglk | ΔavtA | Replacing the start codon of ilvE from ATG to GTG | ΔldhA | ΔpoxB | gltA :: PfliC-gltA | Plasmid pACYCDuet-panBCfbr added

Bars (|) indicate differences between strains.


Plasmids

Strain derived from a randomly mutated strain.


Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
(R)-pantothenate Titer 3.71 g/L 16.95 OD600* Flask [233]
(R)-pantothenate Volumetric productivity 0.08 g/L/h 16.95 OD600* Flask [233]
(R)-pantothenate Titer 1.225 g/L * 19.105 OD600* Flask [233]
(R)-pantothenate Biomass-specific titer 0.058 g/L/OD600 19.105 OD600* Flask [233]
(R)-pantothenate Biomass-specific titer 0.211 g/L/OD600 * 16.95 OD600* Flask [233]
(R)-pantothenate Titer 3.2 g/L * 36 h * Flask [233]
(R)-pantothenate Titer 3.672 g/L * 48 h * Flask [233]
(R)-pantothenate Titer 3.562 g/L * 60 h * Flask [233]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References