Escherichia coli PA119
Ancestors
Derived strains
Genotype with respect to parental
gapC :: gapCcac
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Grows vigorously in the presence of β-alanine | ilvG :: PM1-93-alsScgl, panBC :: PM1-93-panBC | Δglk | ΔavtA | Replacing the start codon of ilvE from ATG to GTG | ΔldhA | ΔpoxB | gltA :: PfliC-gltA | yjiP :: panBC | thrW :: panBC | ykgH :: PM1-93-ilvD | ypjC :: PM1-93-panE | gapC :: gapCcacBars (|) indicate differences between strains.
Strain derived from a randomly mutated strain.
Modifications
| Gene | Organism | Modification |
|---|---|---|
|
Silencing
|
||
|
Overexpression
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| (R)-pantothenate | Biomass-specific titer | 0.241 g/L/OD600 * | 17.227 OD600* | Flask | [233] | ||
| (R)-pantothenate | Titer | 4.108 g/L * | 17.227 OD600* | Flask | [233] | ||
| (R)-pantothenate | Titer | 4.21 g/L * | 48 h * | Flask | [233] | ||
| (R)-pantothenate | Titer | 4.33 g/L * | 60 h * | Flask | [233] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.