Escherichia coli ita12

Ancestors

Derived strains

Genotype with respect to parental

∆pykA ∆pykF::cam


Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pCadCs added | ΔaceA | ∆sucCD | ∆pykA ∆pykF::cam

Bars (|) indicate differences between strains.


Plasmids

  • pCadCs (BBa_J23100_cadA_gltA_T, ColE1 origin, (T=Terminator (BBa_0010)))


Modifications
Gene Organism Modification
Silencing
Silencing

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
itaconate Titer 0.02 g/L Flask [234]
itaconate Substrate yield 0.006 mol/mol of substrate Flask [234]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References