Escherichia coli ita23

Ancestors

Genotype with respect to parental

∆Picd::cam_P2 (BBa_J23115)


Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pCadCs added | ΔaceA | ∆sucCD | ∆pykA ∆pykF::cam | ∆pta | ∆Picd::cam_P2 (BBa_J23115)

Bars (|) indicate differences between strains.


Plasmids

  • pCadCs (BBa_J23100_cadA_gltA_T, ColE1 origin, (T=Terminator (BBa_0010)))


Modifications
Gene Organism Modification
icd
Attenuation

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
itaconate Titer 2.27 g/L 49 h Flask [234]
itaconate Substrate yield 0.767 mol/mol of substrate 49 h Flask [234]
itaconate Titer 32.0 g/L Fed-Batch [234]
itaconate Substrate yield 0.68 mol/mol of substrate Fed-Batch [234]
itaconate Volumetric productivity 0.45 g/L/h Fed-Batch [234]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References