Escherichia coli MEC626/pZE12-cimA

Ancestors

Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔleuC Δ(ackA-pta) ΔpoxB::Kan ΔgltA | ΔKan | ΔgltA::gltA[F383M]-Kan | Plasmid pZE12-cimA added

Bars (|) indicate differences between strains.


Plasmids

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
(R)-citramalate Titer 60.0 g/L 132 h Fed-Batch [235]
(R)-citramalate Substrate yield 0.53 g/g of sustrate 132 h Fed-Batch [235]
(R)-citramalate Titer 1.81 g/L 24 h Flask [235]
(R)-citramalate Substrate yield 0.57 g/g of sustrate 24 h Flask [235]
(R)-citramalate Titer 11.0 g/L 48 h * Batch [235]
acetate Titer 0.75 g/L 48 h * Batch [235]
(R)-citramalate Substrate yield 0.37 g/g of sustrate 48 h Batch [235]
acetate Titer 3.1 g/L 132 h Fed-Batch [235]
(R)-lactate Titer 1.3 g/L 132 h Fed-Batch [235]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References