Escherichia coli W3110 trpD9923/pJLBaroGfbrtktA/pTrc-ant3

Ancestors

Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | trpD9923 | Plasmid pJLBaroGfbrtktA added | Plasmid pTrc-ant3 added

Bars (|) indicate differences between strains.


Plasmids

Strain derived from a randomly mutated strain.


Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
catechol Titer 4.47 g/L 6.92 g/L Fed-Batch [236]
catechol Substrate yield 16.0 % 6.92 g/L Fed-Batch [236]
catechol Titer 0.41 g/L 1.83 g/L 74 h * Flask [236]
anthranilate Titer 0.15 g/L 1.83 g/L 74 h * Flask [236]
catechol Substrate yield 0.1 g/g of sustrate 1.83 g/L 74 h * Flask [236]
catechol Biomass-specific productivity 0.07 g/g DCW/h 1.83 g/L 74 h * Flask [236]
anthranilate Biomass-specific productivity 0.07 g/g DCW/h 1.83 g/L 74 h Flask [236]
catechol Titer 2.51 g/L 10.44 g/L Fed-Batch [236]
catechol Substrate yield 0.07 g/g of sustrate 10.44 g/L Fed-Batch [236]
catechol Biomass-specific productivity 0.02 g/g DCW/h 10.44 g/L Fed-Batch [236]
anthranilate Substrate yield 0.09 g/g of sustrate 10.44 g/L Fed-Batch [236]
anthranilate Biomass-specific productivity 0.02 g/g DCW/h 10.44 g/L Fed-Batch [236]
catechol Titer 2.81 g/L 7.13 g/L Fed-Batch [236]
catechol Substrate yield 0.08 g/g of sustrate 7.13 g/L Fed-Batch [236]
catechol Biomass-specific productivity 0.02 g/g DCW/h 7.13 g/L Fed-Batch [236]
anthranilate Biomass-specific productivity 0.02 g/g DCW/h 7.13 g/L Fed-Batch [236]
anthranilate Substrate yield 0.09 g/g of sustrate 7.13 g/L Fed-Batch [236]
anthranilate Titer 2.12 g/L 7.13 g/L Fed-Batch [236]
catechol Biomass-specific productivity 0.02 g/g DCW/h 6.92 g/L Fed-Batch [236]
anthranilate Titer 2.89 g/L 6.92 g/L Fed-Batch [236]
anthranilate Substrate yield 0.1 g/g of sustrate 6.92 g/L Fed-Batch [236]
anthranilate Biomass-specific productivity 0.07 g/g DCW/h 6.92 g/L Fed-Batch [236]
catechol Titer 1.83 g/L 6.75 g/L Fed-Batch [236]
catechol Substrate yield 0.06 g/g of sustrate 6.75 g/L Fed-Batch [236]
catechol Biomass-specific productivity 0.01 g/g DCW/h 6.75 g/L Fed-Batch [236]
anthranilate Titer 2.21 g/L 6.75 g/L Fed-Batch [236]
anthranilate Substrate yield 0.09 g/g of sustrate 6.75 g/L Fed-Batch [236]
anthranilate Biomass-specific productivity 0.01 g/g DCW/h 6.75 g/L Fed-Batch [236]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References