Escherichia coli trpD9923 PTS-/pTrc-ant3
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | trpD9923 | ΔptsHI, crr::kmR | Plasmid pTrc-ant3 addedBars (|) indicate differences between strains.
Plasmids
-
pTrc-ant3 (Ptrc-antABC(P. aeruginosa PAO1))
Strain derived from a randomly mutated strain.
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| catechol | Titer | 0.37 g/L | 1.58 g/L | 74 h * | Flask | [236] | |
| anthranilate | Titer | 0.22 g/L | 1.58 g/L | 74 h * | Flask | [236] | |
| catechol | Substrate yield | 0.09 g/g of sustrate | 1.58 g/L | 74 h * | Flask | [236] | |
| catechol | Biomass-specific productivity | 0.03 mg/g DCW/h | 1.58 g/L | 74 h * | Flask | [236] | |
| anthranilate | Biomass-specific productivity | 0.03 g/g DCW/h | 1.58 g/L | 74 h * | Flask | [236] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.
References
- Balderas-Hernández, V.E., Treviño-Quintanilla, L.G., Hernández-Chávez, G. et al. Catechol biosynthesis from glucose in Escherichia coli anthranilate-overproducer strains by heterologous expression of anthranilate 1,2-dioxygenase from Pseudomonas aeruginosa PAO1. Microb Cell Fact 13, 136 (2014). https://doi.org/10.1186/s12934-014-0136-x