Silencing
Overexpression
Feedback insensitive
Attenuation
Feed-forward insensitive
Inhibition insensitive
Unstated
Combined modifications

Titer in Flask for 5-hydroxy-L-tryptophan

Modifications
Strain Maximum production tnaAEc folEEc tphDj folXEc folMEc phhBPa nudBEc queDEc folEABs folESg folEZm aroHEc trpEEc trpDEc trpCEc trpBEc trpAEc PTPSHs SPRHs PCBDHs DHPRHs TPH2Hs mlcEc trpLEc aroGEc serAEc gdhEs mtrEc tnaBEc trpREc phhACt
E. coli HTP-1 18.7 mg/L
E. coli HTP-8 22.3 mg/L
E. coli HTP-7 22.5 mg/L
E. coli HTP-5 23.1 mg/L
E. coli HTP-4 23.7 mg/L
E. coli HTP-3 24 mg/L
E. coli HTP-2 27.5 mg/L
E. coli HTP-6 29.9 mg/L
E. coli HTP-9 30.03 mg/L
E. coli HTP-15 31.4 mg/L
E. coli HTP-10 41.35 mg/L
E. coli HTP-14 51.7 mg/L
E. coli HTP-12 56.7 mg/L
E. coli HTP-11 57.2 mg/L
E. coli HTP-13 72.6 mg/L
E. coli HTP-16 78.83 mg/L
E. coli TrpD-Pl/pACJ23-CtAAAH-F 100.4 mg/L
E. coli HTP-17 122.3 mg/L
E. coli TRP93/pSCHTP-LMT 290 mg/L
E. coli TRP46/pSCHTP-LMT 370 mg/L
E. coli TRP0/pETHTP-LMT 400 mg/L
E. coli TRP37/pSCHTP-LMT 540 mg/L
E. coli TRP30/pSCHTP-LMT 570 mg/L
E. coli TRP0/pACHTP-LMT 920 mg/L
E. coli HTP04 1,020 mg/L
E. coli HTP07 1,150 mg/L
E. coli TRP12/pSCHTP-LMT 1,190 mg/L
E. coli HTP101-LMT 1,210 mg/L
E. coli TRP0/pSCHTP-LMT 1,280 mg/L
E. coli TRPmut/pSCHTP-LMT 1,610 mg/L
E. coli HTP08 1,710 mg/L
E. coli HTP05 2,120 mg/L
E. coli HTP14 2,180 mg/L
E. coli HTP06 2,670 mg/L
E. coli HTP13 2,770 mg/L
E. coli HTP12 2,890 mg/L
E. coli HTP09 3,100 mg/L
E. coli HTP10 3,200 mg/L
E. coli HTP11 3,540 mg/L

Titer in Fed-Batch for 5-hydroxy-L-tryptophan

Modifications
Strain Maximum production tnaAEc mlcEc folEABs PTPSHs SPRHs PCBDHs DHPRHs trpLEc trpEEc aroGEc serAEc gdhEs TPH2Hs mtrEc tnaBEc trpDEc trpCEc trpBEc trpAEc trpREc phhACt
E. coli TrpD-PI/pACJ23-CtAAAH-LC 962 mg/L
E. coli HTP11 8,580 mg/L

Substrate yield in Fed-Batch for 5-hydroxy-L-tryptophan

Modifications
Strain Maximum production tnaAEc mlcEc folEABs PTPSHs SPRHs PCBDHs DHPRHs trpLEc trpEEc aroGEc serAEc gdhEs TPH2Hs
E. coli HTP11 0.095 g/g of sustrate

Substrate yield in Flask for 5-hydroxy-L-tryptophan

Modifications
Strain Maximum production tnaAEc mlcEc folEABs PTPSHs SPRHs PCBDHs DHPRHs trpLEc trpEEc TPH2Hs aroGEc serAEc gdhEs
E. coli HTP07 0.031 g/g of sustrate
E. coli HTP08 0.049 g/g of sustrate
E. coli HTP14 0.055 g/g of sustrate
E. coli HTP13 0.073 g/g of sustrate
E. coli HTP12 0.074 g/g of sustrate
E. coli HTP09 0.084 g/g of sustrate
E. coli HTP10 0.084 g/g of sustrate
E. coli HTP11 0.088 g/g of sustrate

Volumetric productivity in Fed-Batch for 5-hydroxy-L-tryptophan

Modifications
Strain Maximum production tnaAEc mlcEc folEABs PTPSHs SPRHs PCBDHs DHPRHs trpLEc trpEEc aroGEc serAEc gdhEs TPH2Hs
E. coli HTP11 480 mg/L/h

Biomass-specific yield in Flask for 5-hydroxy-L-tryptophan

Modifications
Strain Maximum production tnaAEc mlcEc folEABs PTPSHs SPRHs PCBDHs DHPRHs TPH2Hs
E. coli HTP04 58 mg/g DCW
E. coli HTP05 108 mg/g DCW
E. coli HTP06 127 mg/g DCW