Silencing
Overexpression
Feedback insensitive
Attenuation
Feed-forward insensitive
Inhibition insensitive
Unstated
Combined modifications

Titer in Flask for all-trans-lycopene

Modifications
Strain Maximum production crtEPa crtIPa dxsEc pfkAEc pgiEc fbaAEc tpiAEc icdEc mdhEc idiEc gdhAEc gpmAEc gpmBEc ispAEc ppsAEc
E. coli DH5α/pTrc99A, pLyc184 2.52 mg/L
E. coli DH5α/pTrcDx-pfkA, pLyc184 3.25 mg/L
E. coli WLGA/pLyc184 3.71 mg/L
E. coli WLG/pLyc184 3.82 mg/L
E. coli WLAB/pLyc184 4.27 mg/L
E. coli WLA/pLyc184 4.83 mg/L
E. coli DH5α/pTrcDx, pLyc184 4.95 mg/L
E. coli DH5α/pTrcDx-icdA, pLyc184 4.95 mg/L
E. coli DH5α/pTrcDx-pgi, pLyc184 5.32 mg/L
E. coli WLB/pLyc184 5.59 mg/L
E. coli DH5α/pTrcDx-tpiA, pLyc184 6.84 mg/L
E. coli DH5α/pTrcDx-fbaA, pLyc184 6.94 mg/L
E. coli WLGB/pLyc184 8.32 mg/L
E. coli WLGB/pTrcDx-tpiA, pLyc184 8.84 mg/L
E. coli DH5α/pTrcDx-mdh, pLyc184 9.06 mg/L
E. coli DH5α/pTrcDx-idi-tpiA, pLyc184 10.52 mg/L
E. coli DH5α/pTrcDx-idi-fbaA, pLyc184 11.5 mg/L
E. coli DH5α/pTrcDx-idi, pLyc184 12.85 mg/L
E. coli DH5α/pTrcDx-idi-mdh, pLyc184 13.2 mg/L
E. coli WLGB-RPP/pTrcDx-idi-mdh, pLyc184 19.35 mg/L
E. coli WLGB-RPP/pLyc184 19.58 mg/L
E. coli WLGB-RP/pLyc184 19.78 mg/L
E. coli WLGB-RP/pTrcDx-idi-mdh, pLyc184 23.97 mg/L
E. coli WLGB/pTrcDx-idi, pLyc184 25.46 mg/L
E. coli WLAB/pTrcDx-idi-mdh, pLyc184 26.77 mg/L

Titer in Fed-Batch for all-trans-lycopene

Modifications
Strain Maximum production gdhAEc gpmBEc ispAEc dxsEc idiEc crtEPa crtIPa mdhEc ppsAEc
E. coli WLGB-RP/pTrcDx-idi-mdh, pLyc184 206.7 mg/L
E. coli WLGB-RPP/pTrcDx-idi-mdh, pLyc184 283 mg/L

Biomass-specific yield in Flask for all-trans-lycopene

Modifications
Strain Maximum production crtEPa crtIPa dxsEc pfkAEc pgiEc fbaAEc tpiAEc icdEc mdhEc idiEc gdhAEc gpmAEc gpmBEc ispAEc ppsAEc
E. coli DH5α/pTrc99A, pLyc184 1.48 mg/g DCW
E. coli DH5α/pTrcDx-pfkA, pLyc184 1.63 mg/g DCW
E. coli WLG/pLyc184 1.83 mg/g DCW
E. coli WLGA/pLyc184 1.85 mg/g DCW
E. coli WLAB/pLyc184 1.99 mg/g DCW
E. coli WLA/pLyc184 2.47 mg/g DCW
E. coli WLB/pLyc184 2.6 mg/g DCW
E. coli DH5α/pTrcDx-pgi, pLyc184 2.73 mg/g DCW
E. coli DH5α/pTrcDx-icdA, pLyc184 2.98 mg/g DCW
E. coli WLGB/pTrcDx-tpiA, pLyc184 3.03 mg/g DCW
E. coli DH5α/pTrcDx, pLyc184 3.09 mg/g DCW
E. coli WLGB/pLyc184 3.65 mg/g DCW
E. coli DH5α/pTrcDx-fbaA, pLyc184 4.62 mg/g DCW
E. coli DH5α/pTrcDx-mdh, pLyc184 4.83 mg/g DCW
E. coli DH5α/pTrcDx-tpiA, pLyc184 4.89 mg/g DCW
E. coli WLGB/pTrcDx-idi, pLyc184 7.08 mg/g DCW
E. coli DH5α/pTrcDx-idi-tpiA, pLyc184 7.51 mg/g DCW
E. coli DH5α/pTrcDx-idi-fbaA, pLyc184 7.67 mg/g DCW
E. coli WLAB/pTrcDx-idi-mdh, pLyc184 8.5 mg/g DCW
E. coli DH5α/pTrcDx-idi, pLyc184 8.57 mg/g DCW
E. coli WLGB-RP/pLyc184 8.8 mg/g DCW
E. coli WLGB-RPP/pLyc184 9.41 mg/g DCW
E. coli DH5α/pTrcDx-idi-mdh, pLyc184 9.98 mg/g DCW
E. coli WLGB-RPP/pTrcDx-idi-mdh, pLyc184 10.63 mg/g DCW
E. coli WLGB-RP/pTrcDx-idi-mdh, pLyc184 12.32 mg/g DCW

Biomass-specific productivity in Flask for all-trans-lycopene

Modifications
Strain Maximum production crtEPa crtIPa dxsEc pfkAEc pgiEc fbaAEc tpiAEc icdEc mdhEc idiEc gdhAEc gpmAEc gpmBEc ispAEc ppsAEc
E. coli DH5α/pTrc99A, pLyc184 0.037 mg/L/g DCW
E. coli WLG/pLyc184 0.03803 mg/L/g DCW
E. coli WLGA/pLyc184 0.03847 mg/L/g DCW
E. coli DH5α/pTrcDx-pfkA, pLyc184 0.04075 mg/L/g DCW
E. coli WLAB/pLyc184 0.04151 mg/L/g DCW
E. coli WLA/pLyc184 0.05137 mg/L/g DCW
E. coli WLB/pLyc184 0.0542 mg/L/g DCW
E. coli DH5α/pTrcDx-pgi, pLyc184 0.06825 mg/L/g DCW
E. coli DH5α/pTrcDx-icdA, pLyc184 0.0745 mg/L/g DCW
E. coli WLGB/pLyc184 0.07595 mg/L/g DCW
E. coli DH5α/pTrcDx, pLyc184 0.07725 mg/L/g DCW
E. coli DH5α/pTrcDx-fbaA, pLyc184 0.1155 mg/L/g DCW
E. coli DH5α/pTrcDx-mdh, pLyc184 0.12075 mg/L/g DCW
E. coli DH5α/pTrcDx-tpiA, pLyc184 0.12225 mg/L/g DCW
E. coli WLGB-RP/pLyc184 0.18338 mg/L/g DCW
E. coli WLGB/pTrcDx-tpiA, pLyc184 0.18408 mg/L/g DCW
E. coli DH5α/pTrcDx-idi-tpiA, pLyc184 0.18775 mg/L/g DCW
E. coli DH5α/pTrcDx-idi-fbaA, pLyc184 0.19175 mg/L/g DCW
E. coli WLGB-RPP/pLyc184 0.19819 mg/L/g DCW
E. coli DH5α/pTrcDx-idi, pLyc184 0.21425 mg/L/g DCW
E. coli WLGB-RPP/pTrcDx-idi-mdh, pLyc184 0.22152 mg/L/g DCW
E. coli DH5α/pTrcDx-idi-mdh, pLyc184 0.2495 mg/L/g DCW
E. coli WLGB-RP/pTrcDx-idi-mdh, pLyc184 0.25663 mg/L/g DCW
E. coli WLGB/pTrcDx-idi, pLyc184 0.53035 mg/L/g DCW
E. coli WLAB/pTrcDx-idi-mdh, pLyc184 0.55773 mg/L/g DCW