Silencing
Overexpression
Feedback insensitive
Attenuation
Feed-forward insensitive
Inhibition insensitive
Unstated
Combined modifications
Titer in Flask for serotonin
| Modifications | ||||
|---|---|---|---|---|
| Strain | Maximum production | T5HOs | TDCCr | TDCOs |
| E. coli BL21(DE3)/pET300-T5H | 0 mg/L | |||
| E. coli BL21(DE3)/pET300-∆37T5H-RR | 0 mg/L | |||
| E. coli/pET28b | 0 mg/L | |||
| E. coli BL21(DE3)/pCOLADuet-GST∆37T5H+TDC | 24 mg/L | |||
| E. coli/pET28b-TDC | 35 mg/L | |||
Titer in Fed-Batch for serotonin
| Modifications | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Strain | Maximum production | mtrEc | tnaAEc | tnaBEc | aroGEc | serAEc | trpEEc | trpDEc | trpCEc | trpBEc | trpAEc | trpREc | PCBDHs | DHPRHs | phhACt | TDCCr |
| E. coli TrpD-Pl / pCtAAAH-LC / pCOLAJ23-TDC.2 | 0 mg/L | |||||||||||||||
| E. coli TrpD-Pl / pCtAAAH-LC / pBAD-TDC | 0.8 mg/L | |||||||||||||||