L-serine charts

Silencing

Overexpression

Feedback insensitive

Attenuation

Feed-forward insensitive

Inhibition insensitive

Unstated

Combined modifications

Titer in Batch for L-serine

		Modifications
Strain	Maximum production	sdaA_Ec	iclR_Ec	arcA_Ec	serA_Ec	serB_Ec	serC_Ec
E. coli DH5α	1,350 mg/L
E. coli YF-3	1,520 mg/L
E. coli YF-4	2,030 mg/L
E. coli YF-5	2,290 mg/L
E. coli YF-6	2,510 mg/L
E. coli YF-8	3,700 mg/L
E. coli YF-7	4,500 mg/L

Titer in Fed-Batch for L-serine

		Modifications
Strain	Maximum production	sdaA_Ec	iclR_Ec	arcA_Ec	serA_Ec	serB_Ec	serC_Ec	sdaB_Ec	tdcG_Ec	glyA_Ec	eamA_Ec	mntS_Ec	sdaC_Ec	sstT_Ec	tdcC_Ec	pgk_Ec	cycA_Ec
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACYCDuet-1-serB-eamA-c-His)	6,690 mg/L
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)	8,300 mg/L
E. coli YF-7	8,340 mg/L
E. coli Q1 (DE3) thrA S357R (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)	9,000 mg/L
E. coli Q3 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)	9,080 mg/L
E. coli ES-1/pSC-08	23,800 mg/L
E. coli ES-1234/pSC-08	26,300 mg/L
E. coli ES-13/pSC-08	29,600 mg/L
E. coli ES-134/pSC-08	34,800 mg/L
E. coli ALE-5	37,300 mg/L

Titer in Flask for L-serine

		Modifications
Strain	Maximum production	serC_Ec	serB_Ec	eamA_Ec	sdaA_Ec	serA_Ec	sdaB_Ec	tdcG_Ec	glyA_Ec	gcvH_Ec	gcvP_Ec	gcvT_Ec	pgk_Ec	serA_Cg	sdaC_Ec	cycA_Ec	sstT_Ec	tdcC_Ec	tnaA_Ec	cyuA_Ec
E. coli E3 (pPK10)	83.3 mg/L
E. coli ES/pSC-08	129 mg/L
E. coli E4G1 (pPK10)	137.1 mg/L
E. coli ES-1/pSC-08	220 mg/L
E. coli E4G2 (pPK10)	266.3 mg/L
E. coli ES-1234/pSC-08	384 mg/L
E. coli ES-13/pSC-08	385 mg/L
E. coli ES-134/pSC-08	445 mg/L
E. coli S1 (DE3) (pCDF-Duet-serAmut-serC pACTCDuet-1-serB)	490 mg/L
E. coli T1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)	530 mg/L
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)	890 mg/L
E. coli BW08	901.3 mg/L
E. coli (pBCBA, pJD760)	3,800 mg/L

Substrate yield in Flask for L-serine

		Modifications
Strain	Maximum production	serC_Ec	serB_Ec	eamA_Ec	sdaA_Ec	serA_Ec
E. coli (pBCBA, pJD760)	0.24 g/g of sustrate
E. coli S1 (DE3) (pCDF-Duet-serAmut-serC pACTCDuet-1-serB)	0.245 g/g of sustrate

Substrate yield in Fed-Batch for L-serine

		Modifications
Strain	Maximum production	sdaA_Ec	sdaB_Ec	tdcG_Ec	glyA_Ec	serA_Ec	serC_Ec	serB_Ec	eamA_Ec	mntS_Ec	sdaC_Ec	sstT_Ec	tdcC_Ec	pgk_Ec	cycA_Ec
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACYCDuet-1-serB-eamA-c-His)	0.1 g/g of sustrate
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)	0.12 g/g of sustrate
E. coli Q3 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)	0.18 g/g of sustrate
E. coli ALE-5	0.24 g/g of sustrate
E. coli ES-1/pSC-08	0.25 g/g of sustrate
E. coli ES-1234/pSC-08	0.29 g/g of sustrate
E. coli ES-13/pSC-08	0.31 g/g of sustrate
E. coli ES-134/pSC-08	0.32 g/g of sustrate

Biomass-specific titer in Flask for L-serine

		Modifications
Strain	Maximum production	sdaA_Ec	serA_Ec	serC_Ec	serB_Ec	sdaB_Ec	tdcG_Ec
E. coli S1 (DE3) (pCDF-Duet-serAmut-serC pACTCDuet-1-serB)	730
E. coli T1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)	1,940

Volumetric productivity in Flask for L-serine

		Modifications
Strain	Maximum production	sdaA_Ec	sdaB_Ec	tdcG_Ec	glyA_Ec	serA_Ec	serC_Ec	serB_Ec
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)	2,730 mg/L/h

Volumetric productivity in Fed-Batch for L-serine

		Modifications
Strain	Maximum production	sdaA_Ec	sdaB_Ec	tdcG_Ec	glyA_Ec	serA_Ec	serC_Ec	serB_Ec	eamA_Ec	mntS_Ec
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACYCDuet-1-serB-eamA-c-His)	130 mg/L/h
E. coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)	180 mg/L/h
E. coli Q3 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)	180 mg/L/h
E. coli ALE-5	830 mg/L/h