Silencing
Overexpression
Feedback insensitive
Attenuation
Feed-forward insensitive
Inhibition insensitive
Unstated
Combined modifications

Titer in Flask for (-)-patchoulol

Modifications
Strain Maximum production atoBEc ERG13Sc HMG1Sc ERG12Sc ERG8Sc MVD1Sc idiEc ispAEc PTS2Pc PTS1Pc ackAEc ptaEc ldhAEc adhEEc frdAEc macAEc macBEc msbAEc
E. coli PTS1 0.11 mg/L
E. coli PTS2 14 mg/L
E. coli 010H (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A) 168 mg/L
E. coli 040H (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A) 238 mg/L
E. coli 050H (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A) 321.4 mg/L
E. coli 060HA (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A) 338.6 mg/L

Titer in Fed-Batch for (-)-patchoulol

Modifications
Strain Maximum production ackAEc ptaEc ldhAEc adhEEc frdAEc macAEc macBEc msbAEc atoBEc ERG13Sc HMG1Sc ERG12Sc ERG8Sc MVD1Sc idiEc ispAEc PTS2Pc
E. coli 060HA (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A) 970.1 mg/L

Biomass-specific yield in Flask for (-)-patchoulol

Modifications
Strain Maximum production ackAEc ptaEc ldhAEc adhEEc frdAEc macAEc macBEc msbAEc atoBEc ERG13Sc HMG1Sc ERG12Sc ERG8Sc MVD1Sc idiEc ispAEc PTS2Pc
E. coli 010H (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A) 16.04 mg/g DCW
E. coli 040H (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A) 21.4 mg/g DCW
E. coli 050H (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A) 25.63 mg/g DCW
E. coli 060HA (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A) 48.6 mg/g DCW

Volumetric productivity in Flask for (-)-patchoulol

Modifications
Strain Maximum production ackAEc ptaEc ldhAEc adhEEc frdAEc macAEc macBEc msbAEc atoBEc ERG13Sc HMG1Sc ERG12Sc ERG8Sc MVD1Sc idiEc ispAEc PTS2Pc
E. coli 060HA (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A) -999,999,999 mg/L/h

Volumetric productivity in Fed-Batch for (-)-patchoulol

Modifications
Strain Maximum production ackAEc ptaEc ldhAEc adhEEc frdAEc macAEc macBEc msbAEc atoBEc ERG13Sc HMG1Sc ERG12Sc ERG8Sc MVD1Sc idiEc ispAEc PTS2Pc
E. coli 060HA (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A) -999,999,999 mg/L/h