Zhi-Jun Zhao, Chun Zou, Yi-Xing Zhu, Jun Dai, Sheng Chen, Dan Wu, Jing Wu & Jian Chen (2011). Development of l-tryptophan production strains by defined genetic modification in Escherichia coli. Journal of Industrial Microbiology & Biotechnology.
Strains
- Escherichia coli W3110/pSV02
- Escherichia coli FB-03
- Escherichia coli FB-01
- Escherichia coli FB-02
- Escherichia coli W3110/pSV01
- Escherichia coli W3110/pSV-aroFfbr-trpEfbrD
- Escherichia coli FB-02/pSV03
- Escherichia coli FB-04
- Escherichia coli FB-01/pSV03
- Escherichia coli FB-03/pSV03
- Escherichia coli FB-04/pSV03
Plasmids
Metabolite production
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L-tryptophan
-
Titer
- 13.3 g/L (Escherichia coli FB-04/pSV03 in Fed-Batch)
- 10.8 g/L (Escherichia coli FB-03/pSV03 in Fed-Batch)
- 7.8 g/L (Escherichia coli FB-02/pSV03 in Fed-Batch)
- 2.8 g/L (Escherichia coli FB-01/pSV03 in Fed-Batch) Substrate yield
- 0.1 g/g of sustrate (Escherichia coli FB-04/pSV03 in Fed-Batch)
- 0.07 g/g of sustrate (Escherichia coli FB-03/pSV03 in Fed-Batch)
- 0.05 g/g of sustrate (Escherichia coli FB-02/pSV03 in Fed-Batch)
- 0.02 g/g of sustrate (Escherichia coli FB-01/pSV03 in Fed-Batch)