Qingyang Xu, Fang Bai, Ning Chen & Gang Bai (2017). Gene modification of the acetate biosynthesis pathway in Escherichia coli and implementation of the cell recycling technology to increase L-tryptophan production. PLOS ONE.

Strains

• Escherichia coli TRTHB
• Escherichia coli TRTHBPAB
• Escherichia coli TRTHBB
• Escherichia coli TRTHBPA
• Escherichia coli TRTHBPT

Metabolite production

• acetate
  Titer
  • 1.51 g/L (Escherichia coli TRTHB in Fed-Batch)
  • 1.25 g/L (Escherichia coli TRTHBPA in Fed-Batch)
  • 1.41 g/L (Escherichia coli TRTHBB in Fed-Batch)
  • 1.18 g/L (Escherichia coli TRTHBPAB in Fed-Batch)
  • 0.87 g/L (Escherichia coli TRTHBPA in Fed-Batch)
  • 1.33 g/L (Escherichia coli TRTHBPT in Fed-Batch)
• L-tryptophan
  Titer
  • 39.61 g/L (Escherichia coli TRTHBPAB in Fed-Batch)
  • 39.12 g/L (Escherichia coli TRTHB in Fed-Batch)
  • 47.18 g/L (Escherichia coli TRTHBPA in Fed-Batch)
  • 55.12 g/L (Escherichia coli TRTHBPA in Fed-Batch)
  Substrate yield
  • 15.67 % (Escherichia coli TRTHBPAB in Fed-Batch)
  • 14.89 % (Escherichia coli TRTHB in Fed-Batch)
  • 17.83 % (Escherichia coli TRTHBPA in Fed-Batch)
  • 19.75 % (Escherichia coli TRTHBPA in Fed-Batch)
  • 17.42 % (Escherichia coli TRTHBPT in Fed-Batch)
  • 16.05 % (Escherichia coli TRTHBB in Fed-Batch)
  • 19.27 % (Escherichia coli Dmtr in Fed-Batch)
  • 19.45 % (Escherichia coli Dmtr-Y in Fed-Batch)