Hu, Miaomiao; Li, Mengli; Li, Chenchen & Zhang, Tao. Biosynthesis of Lacto-N-fucopentaose I in Escherichia coli by metabolic pathway rational design. Carbohydrate Polymers. 2022, 297, 120017.

Strains

• Escherichia coli BZ
• Escherichia coli BZW
• Escherichia coli BL21(DE3)

Plasmids

• pACYDuet-1
• pCOLDuet-1
• CloDF13 ori, lacI, P_T7lac-lgtA, p_T7lac-wbgO
• pET-pgi
• pET-pgm
• pET-glmM
• pET-glmU
• pET-glmS
• pET-glmUS
• pET-glmM-glmUS
• pET-galE
• pET-galT
• pET-galK
• pET-galET
• galETK
• pET-galETK-futC
• pACY-futC
• pCDFDuet-1
• pETDuet-1