Dengwei Lei, Zetian Qiu, Jihua Wu, Bin Qiao, Jianjun Qiao & Guang-Rong Zhao (2021). Combining Metabolic and Monoterpene Synthase Engineering for de Novo Production of Monoterpene Alcohols in Escherichia coli. ACS Synthetic Biology.
Strains
- Escherichia coli BW25113(DE3)
- Escherichia coli EIP81
- Escherichia coli ENP
- Escherichia coli ENO1
- Escherichia coli ENB1
- Escherichia coli ENB3
- Escherichia coli ENB14
- Escherichia coli ENB27
- Escherichia coli ENB33
- Escherichia coli ENB36
- Escherichia coli ENB41
- Escherichia coli ENB48
- Escherichia coli ENB49
- Escherichia coli ENB50
- Escherichia coli ENB51
- Escherichia coli ENB52
- Escherichia coli ENB53
- Escherichia coli ENB54
- Escherichia coli ENB55
- Escherichia coli ENB56
- Escherichia coli ENB58
- Escherichia coli ENO2
- Escherichia coli ENB59
- Escherichia coli ENB60
- Escherichia coli ENO5
- Escherichia coli ENO6
- Escherichia coli ENO7
- Escherichia coli ENB2
- Escherichia coli ENB57
Plasmids
- pMAP6
- pISP5
- pNLSt1
- pNPPS
- pNO1
- pNB1
- pNB2
- pNB3
- pNB14
- pNB27
- pNB33
- pNB36
- pNB41
- pNB48
- pNB49
- pNB50
- pNB51
- pNB52
- pNB53
- pNB54
- pNB55
- pNB56
- pNB57
- pNB58
- pNO2
- pNB59
- pNB60
- pNO5
- pNO6
- pNO7
Metabolite production
-
nerol
-
Titer
- 55.37 mg/L (Escherichia coli ENO1 in Flask)
- 34.2 mg/L (Escherichia coli ENP in Flask)
- 261.88 mg/L (Escherichia coli ENO2 in Flask)
- 966.55 mg/L (Escherichia coli ENO2 in Flask)
- 430.88 mg/L (Escherichia coli ENO2 in Flask)
- 498.86 mg/L (Escherichia coli ENO2 in Flask)
- 531.68 mg/L (Escherichia coli ENO2 in Flask)
- 283.79 mg/L (Escherichia coli ENB57 in Flask)
- 172.05 mg/L (Escherichia coli ENB57 in Flask)
- 68.77 mg/L (Escherichia coli ENB36 in Flask)
- 28.2 mg/L (Escherichia coli ENB1 in Flask)
- 15.7 mg/L (Escherichia coli ENB2 in Flask)
- 36.1 mg/L (Escherichia coli ENB3 in Flask)
- 11.04 mg/L (Escherichia coli ENB27 in Flask)
- 13.07 mg/L (Escherichia coli ENB48 in Flask)
- 21.18 mg/L (Escherichia coli ENB49 in Flask)
- 2.43 mg/L (Escherichia coli ENB50 in Flask)
- 45.49 mg/L (Escherichia coli ENB51 in Flask)
- 30.8 mg/L (Escherichia coli ENB52 in Flask)
- 33.33 mg/L (Escherichia coli ENB53 in Flask)
- 47.01 mg/L (Escherichia coli ENB54 in Flask)
- 22.68 mg/L (Escherichia coli ENB55 in Flask)
- 25.73 mg/L (Escherichia coli ENB56 in Flask)
- 185.82 mg/L (Escherichia coli ENB57 in Flask)
- 55.12 mg/L (Escherichia coli ENB58 in Flask) Volumetric productivity
- 12.39 mg/L/h (Escherichia coli ENO2 in Flask)
-
(+)-borneol
-
Titer
- 0.0 mg/L (Escherichia coli ENB1 in Flask)
- 4.26 mg/L (Escherichia coli ENB2 in Flask)
- 0.62 mg/L (Escherichia coli ENB3 in Flask)
- 10.27 mg/L (Escherichia coli ENB27 in Flask)
- 28.26 mg/L (Escherichia coli ENB57 in Flask)
- 32.67 mg/L (Escherichia coli ENB57 in Flask)
- 38.46 mg/L (Escherichia coli ENB57 in Flask)
- 74.09 mg/L (Escherichia coli ENB57 in Flask)
- 87.2 mg/L (Escherichia coli ENB57 in Flask)
- 9.8 mg/L (Escherichia coli ENB48 in Flask)
- 7.9 mg/L (Escherichia coli ENB49 in Flask)
- 1.8 mg/L (Escherichia coli ENB50 in Flask)
- 0.0 mg/L (Escherichia coli ENB51 in Flask)
- 2.32 mg/L (Escherichia coli ENB52 in Flask)
- 0.0 mg/L (Escherichia coli ENB53 in Flask)
- 11.4 mg/L (Escherichia coli ENB54 in Flask)
- 16.35 mg/L (Escherichia coli ENB55 in Flask)
- 11.2 mg/L (Escherichia coli ENB56 in Flask)
- 5.07 mg/L (Escherichia coli ENB58 in Flask)